Determination of the Isoniazid N-Acetylation Phenotype in a Sample Indian Population

An isoniazid N-acetylation phenotyping study was carried out in 200 human volunteers. A single urine sample taken 5 h post-isoniazid oral dose of 300 mg analyzed and urinary concentrations its metabolite acetylisoniazid were determined by high-performance liquid chromatography. Acetylator phenotype from the metabolic ratio N-acetylisoniazid to concentration sample. The cut-off value, antimode, distinguish between slow fast acetylators probit analysis. Of total 194 subjects phenotyped, 70.1 % acetylators, 27.6 intermediate 2.7 acetylators.